بررسی تأثیر پروبیوتیک لاکتوباسیلوس بولگاریکوس (Lactobacillus bulgaricus) ریزپوشانی شده با نانوذرات آلژینات/کیتوزان بر رشد و کارایی تغذیه در فیل ماهی جوان (Huso huso)

نوع مقاله : بهداشت و بیماری های آبزیان

نویسندگان

1 گروه بهداشت آبزیان، دانشکده دامپزشکی دانشگاه شهید چمران اهواز، اهواز، ایران

2 گروه علوم درمانگاهی، دانشکده دامپزشکی دانشگاه شهید چمران اهواز ، اهواز، ایران

3 مرکز تحقیقات آبزی پروری ایران، مرکز تحقیقات ذخایر آبزیان داخلی گرگان، گلستان، ایران

4 مرکز تحقیقات دارویی دانشگاه علوم پزشکی مشهد، دانشکده علوم پزشکی، مشهد، ایران

5 گروه پاتوبیولوژی، دانشکده دامپزشکی دانشگاه شهید چمران اهواز ، اهواز، ایران

چکیده

زمینه مطالعه: در آبزی پروری رشد سریع، کارایی مناسب تغذیه و افزایش مقاومت در برابر بیماری‌ها بسیار مطلوب است. یکی از راهکارهای مناسب برای بهبود شاخص‌های رشد و مقاومت ماهی استفاده از باکتری‌های پروبیوتیکی است. هدف: مطالعه حاضر به منظور ارزیابی اثر پروبیوتیکی باکتری لاکتوباسیلوس بولگاریکوس ریزپوشانی شده با نانوذرات آلژینات/کیتوزان بر رشد و کارایی تغذیه در فیل ماهی جوان (Huso huso) انجام گرفت. روش کار: این آزمایش به صورت طرح کاملاً تصادفی در قالب چهار گروه تیمار و یک گروه شاهد هرکدام  با سه تکرار صورت پذیرفت، برای این منظور تعداد 375 قطعه فیل ماهی با میانگین وزنی  gr8/2±2/27 در پنج گروه تقسیم شدند.تیمار‌های آزمایشی شامل: T1 با غذای حاوی آلژینات/کیتوزان بدون باکتری، T2 با غذای حاوی باکتری ریزپوشانی شده به روش امولسیون، T3 با غذای حاوی باکتری ریزپوشانی شده به روش ژلاسیون یونی، T4 با غذای حاوی باکتری بدون پوشش و گروه شاهد با غذای پایه تغذیه شدند. ماهیان روزانه به میزان 3 % وزن بدن تغذیه شدند. نتایج: نتایج نشان دادند که حداکثرمقدار ضریب تبدیل غذایی (FCR) در T3 (11/0±64/1) و کمترین آن درT4 (06/0±14/1) بود. T4 در شاخص‌های SGR، PER، FER، در روز 30 و 60 دارای بهترین عملکرد نسبت به سایر تیمارها بود. نتیجه‌گیری‌نهایی: براساس نتایج بدست آمده می‌توان نتیجه گرفت که عملکرد رشد در تیمارهایی که کیتوزان و آلژینات به عنوان پوشش حضور داشتند نسبت به تیمار لاکتوباسیلوس بدون پوشش پایین تر بود.

کلیدواژه‌ها

عنوان مقاله [English]

Comparison effects of Lactobasillos bulgaricus microencapsulated by nano alginat/chitosan on Growth performance and Feed efficiency great sturgeon (Huso huso) juveniles.

نویسندگان [English]

  • Seyed Samad Hosseini 1
  • Mojtaba Alishahi 2
  • Koroush Amini 3
  • Mohammadreza Aabbaspour 4
  • Masoud Ghorbanpoor 5
  • Takavar Mohammadian 2
1 1Department of Clinical Sciences, Faculty of Veterinary Medicine Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 2Department of Clinical Sciences, Faculty of Veterinary Medicine Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 3Inland Waters Aquatics Stocks Research Center Gorgan, Gorgan, Iran
4 Targeted Drug Delivery Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
5 5Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran
چکیده [English]

BACKGROUND: In aquaculture the fast growth, high feed efficiency and increased resistance against pathogens are favorable. One of the suitable approaches for improving the growth indices and resistance against pathogens is the application of probiotic bacteria. OBJECTIVES: The present study was conducted to evaluate the effect of nano/microencapsulated Lactobasillus bulgaricus by nano alginate/chitosan on the growth rate and feeding efficiency of great sturgeon (Huso huso) juveniles. METHODS: This experiment was conducted in a completely random design in four groups and group (5) with triplicate. 375 fish weighing (27.28±2.86 gr mean±SD), were randomly divided in to five equal groups in triplicates. The test groups were as follows: fish in T1 were fed with alginate/chitosan without bacterium, fish in T2 were fed with encapsulated bacteria with emulsification method, fish in T3 were fed with encapsulated bacteria with Ionicgelation method, fish in T4 were fed with only bacteria and control group. The fish were fed  3% of body weight per day. RESULTS: The results showed  the highest feed conversion ratio (FCR) was measured in T3 (1.64±0.11) and the lowest was observed in T4 (1.14±0.06).The highest values of SGR, PER, FER in T4 were seen among  the  treatments at days 30 and 60.                                                                                                                                             CONCLUSIONS: According the result, nanoalginat/Chithosan used for encapsulation of probiotics can be lead to decrease in growth performance compared to the other treatments.

کلیدواژه‌ها [English]

  • Probiotic
  • Nanocapsulate
  • Husu huso
  • Growth parameters

مراجع

Allan-Wojtas,  P., Hansen,  L.T., Paulson, A.T.(2008) Microstructural studies of probiotic bacteria-loaded alginate microcapsules using standard electron microscopy techniques and anhydrous fixation. LWT-Food Sci Tech. 41: 101-8.##
Aragon- Alegro, L.C., Alegro, J.A., Cardarelli, H.R., Chiu, M.C., Saad, S.M. (2007) Potentially probiotic and symbiotic chocolate mousse. LWT-Food SciTech. 40: 669-75.##
Bagheri, T., Hedayati, S. A., Yavari, V., Alizade, M., Farzanfar, A. (2008) Growth, Survival and gut Microbial load of Rainbow trout (Onchorhynchus mykiss) fry given diet supplemented with probiotic during the two months of first feeding. Turk J Fish  Aquat Sci. 8: 43-48.##
Balcazar, J.L., Blas, I., Ruiz-Zarzuela, I., Cunningham, D., Vandrell, D. Muzquiz J.L.(2006) Review: The role of probiotics in aquaculture, Vet Microbiol. 114: 173-186.##
Bar-Ilan, O., Albrecht,  R.M., Fako,  V.E., Furgeson,  D.Y.(2009) Toxicity  assessments  of multisized gold  and  silver  nanoparticles  in zebrafish embryos, Small. 5: 1897-1910.##
Blottiere, H.M., Buecher, B., Galmiche, J.P.,  Cherbut, C. (2007) Molecular analysis of the effect of short-chain fatty acids on intestinal cell proliferation. Proceeding Nutri Soci. 62: 101-106.##
Boonyo, W., Junginger,  H.E., Waranuch, N., Polnok, A.,  Pitaksuteepong, T. (2007) Chitosan and trimethylchitosan chloride (TMC) as adjuvants for inducingimmune responses to ovalbumin in mice followingnasal administration. J Cont Release. 121: 168-175.##
Boylston, T.D., Vinderola, C.G., Ghoddusi, H.B., Reinheimer, J.A. (2004) Incorporation of bifidobacteriainto cheeses: challenges and rewards. Int Dairy J. 14: 375-387.##
Chandramouli, V., Kailasapathy, K.P., peireis, J.M.(2004) An improved method of microencapsulationand its evaluation to protect lactobacillus spp. In simulated gastric conditions. J Microb Methods. 56: 27-35.##
Cheba, B.A. (2011) Chitin and chitosan: marine biopolymers with unique properties and versatile applications. Global J Biotech Biochem. 6: 149-153.##
Doherty, S.B., Gee, V.L., Ross, R.P., Stanton, C., Fitzgerald, G.F., Brodkorb, A. (2010) Efficacy of whey protein gel networks, as potential viability- enhancing scaffolds for cell immobilization of Lactobacillus rhamnosus GG. J Microb Methods. 80: 231-241.##
Dutta, P.K., Dutta, J., Tripathi, V.S. (2004) Chitin andchitosan: Chemistry, properties and applications. J Sci Ind Res. 63: 20-31.##
FAO. (2010) The state of world fisheries and aquaculture. NO:944.Rome.243.pp.##
Geng, X., Dong, X., Tan, B., Yang, Q., Chi, S., Liu, H., Liu,  X. (2011) Effects of dietary chitosan and Bacillus subtilis on the growth performance nonspecificimmunity and disease resistance of cobia, Rachycentron canadum. Fish Shellfish Immunol. 31:400- 406.##
Gomez-Gil, B., Roque, A., Turnbull, J.F. (2000) The use andselection of probiotic bacteria for use in the culture of Larvalaquatic organisms. Aquaculture. 191: 259-270.##
Gopalakannan, A.,  Arul, V. (2006)  Immunomodulatory effects of dietary intake of chitin, chitosan and levamisole on the immune system of Cyprinus carpio and control of Aeromonas hydrophila infection in ponds. Aquaculture. 255.: 179-187.##
Harikrishnan, R., Kim, J., Balasundaram, C., Heo, M. (2012) Immunomodulatory effects of chitin andchitosan enriched diets in Epinephelus bruneusagainst Vibrio alginolyticus infection. Aquaculture. 326: 46-52.##
Huiyi, S., Weiting, Y., Meng, G., Xiudong, L., Xiaojun, M. (2013) Microencapsulated probiotics using emulsification technique coupled with internal or external gelation process. J Carbohydr polym. 96: 181-189.##
Ichikawa, H., Kuroiwa, T., Inagaki, A., Shineha, R., Nishihira, T., Satomi, S. Sakata, T. (1999)  Probiotic bacteria stimulate gut epithelial cell proliferation in rat. Digest Dis Sci. 44: 2119-2123.##
IUCN. (1996) IUCN Red List of Threatened Animals. IUCN, Gland, Switzerland and Cambridge, UK.
Jafarian, H., Azari Takami, G., Kamali, A., Soltani, M., and Habibirezaei, M. (2007a) The use of probiotic bacillus bioencapsulated with Artemia urmiana nauplii for the growth and survival in Acipenser persicus larvae. J Agric Sci Nat Reso. 14: 77-87. (In Persian).##
Jafarian, H., Soltani, M., Abedian, A.M. (2007b)The influence some of probiotic bacillus on feeding efficiency and nutrient body composition of Beluga (Huso huso) larvae. J Agri Sci Nat  Reso. 14: 60-71.##
Khalil,  A.H., Mansour, E.H. (1998)  Alginate encapsulated bifidobacteria survival in mayonnaise. J Food Sci 63: 702-5.##
Kono, M., Matsui, T., Shimizu, C.(1987)  Effects of chitin, chitosan and cellulose as dietsupplements on the growth of cultured fish. Nippon Suisan Gakka. 53: 125-129.##
Krasaekoopt, W., Bhandari, B., Deeth, H.(2004) The influence of coating materials on some properties of alginate beads and survivability of microencapsulated probiotic bacteria. Int Dairy J. 14: 737-43.##
Lara-Flores, M., Olvera-Novoa, M.A., Guzman-Mendez, B.E., Lopez-Madrid, W. (2003) Use of the bacteria Streptococcus faecium and Lactobacillus acidophilus, and the yeast Saccharomyces cerevisiae as growth promoters in Nile tilapia (Oreochromis niloticus). Aquaculture. 216: 193-201.##
Lin, S., Pan, Y., Luo, L., Luo, L. (2011) Effects of dietary b-1,3-glucan, chitosan or raffinoseon the growth, innate immunity and resistance of koi (Cyprinus carpio koi). Fish Shellfish Immunol. 31: 788-794.##
Lin, S., Mao, S., Guan, Y., Luo, L.  Pan, Y. (2012) Effects of dietary chitosan oligosaccharidesand Bacillus coagulans on the growth, innateimmunity and resistance of koi (Cyprinus carpiokoi). Aquaculture. 342: 36- 41.##
Maqsood, S., Singh, P., Samoon, M.H., Balange, A.K. (2010) Effect of dietary chitosan on nonspecificimmune response and growth of Cyprinuscarpio challenged with Aeromonas hydrophila. Int Aquat Res. 2: 77-85.##
Maria, A.A., Ana, I.B., Antonio, A.V., Miguel, A.C. (2014) Alginat/chitosan nanopartices for encapsulation and controlled release of vitaminB2. Int J Biol Macromol. 71: 141-146.##
Melinda,  M. (2012)  Dietary Supplements for Improving Body Composition and Reducing Body Weight. Int J Sport Nutri Exer Metabol. 22: 139 -154.##
Mohammadian, T., Alishahi, M. Tabandeh, M.R., Ghorbanpoor, M., Gharibi.D., Tollabi, M., Rohanizadeh, S. (2015) Probiotic effects of Lactobacillus plantarum and L. delbrueckii ssp.bulguricus on some immune-related parameters in Tor grypus. Aquacul Int.##
Mortazavian, A., Razavi, S.H., Ehsani, M.R., Sohrabvandi, S. (2007)  Principles and methods of microencapsulation of probiotic microorganisms. Iran J Biotech. 5: 1-18.##
No, H., Park, N.Y., Lee, S.H., Meyers, S.P. (2002) Antibacterial activity of chitosans and chitosanoligomers with different molecular weights. Int J Food Microb. 74: 65-72.##
Pelicano, E.R.L., Souza, P.A., Souza, H.B.A., Figueiredo, D.F., Boiago, M.M., Carvalho, S.R., Bordon, V.F. (2005) Intestinal mucosa development in broiler chickens fed natural growth promoters. Revista Brasileira de Ciencia Accola. 7: 221-229.##
Picot, A., Lacroix, C. (2004) Encapsulation of bifidobacteriain whey  protein-based microcapsules and survival in simulated gastrointeatinal conditions and in yoghurt. Int Dairy J. 14: 505-515.##
 Pirarat,  N., Pinpimai,  K., Endo,  M., Katagiri, T., Ponpornpisit,  A., Chansue, N.,  Maita, M. (2011) Modulation of intestinal morphology and immunity in nile tilapia (Oreochromis niloticus) by Lactobacillus rhamnosus GG. Res Vet Sci. 91: 92-97. ##
Qin, C., Du, Y., Xiao, L., Li, Z., Gao, X. (2002)  Enzymic preparation of water-soluble chitosan and their antitumor activity. Int J Biol Macromol. 31: 111-117.##
Rokka, S., Rantamaki, P. (2010) Protecting probiotic bacteria by microencapsulation:challenges  for industrial applications. Euro Food Res Tech. 231: 1-12.##
Seferian,  P.G., Martinez,  M.L. (2001) Immunestimulating activity of two new chitosan containingadjuvant formulations. Vaccine. 19: 661-668.##
Sharifzadeh, M.B., Hosseinzadeh,  M.J., Younesi, H. (2012) Whey Processing with nano chitosan, World Appl Sci J. 19: 530-537.##
Shiau, S.Y.,  Yu, Y.P. (1999) Dietary supplementation of chitin and chitosan depressesgrowth in Tilapia, Oreochromis niloticus_O. auratus. Aquaculture. 179: 439-446.##
Tafi, E., Meshkini, S. (2015) The effect of different levels of Chitosan on the growth parameters of Rainbow truot (Onchorhynchus mykiss). J Anim Biol. 4: 35-44.##
Vazquez, J.A., Gonzalez, M.P., Murado, P. (2005)  Effects of lactic acid bacteria cultures on pathogenic microbiota from fish. Aquaculture. 245: 149-161.##
Wang, Y.B., Xu, Z. (2006) Effect of probiotics for common carp (Cyprinus carpio) based on growth performance and digestive enzyme activities. Anim Feed Sci Tech. 127: 283-29.##
Yazdani, A., Shakorian, M., Pourali, H. M., Sayed Hassani, M. H., Paykaran Man, N.,  Yegane, H. (2011)  Extension and rearing of Huso huso for production of meat. Final report. Int Sturgeon Res Insti. p. 57. (In Pesian)##
Yen, M.T., Yang, J. H., Mau, J.L. (2008) Antioxidantproperties of chitosan from crab shells. Carbohydr Polym. 74: 840-844.##
مجله تحقیقات دامپزشکی (Journal of Veterinary Research)
دوره 73، شماره 2
تیر 1397
صفحه 181-190

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