بررسی تأثیر پروبیوتیک لاکتوباسیلوس بولگاریکوس (Lactobacillus bulgaricus) ریزپوشانی شده با نانوذرات آلژینات/کیتوزان بر رشد و کارایی تغذیه در فیل ماهی جوان (Huso huso)

نوع مقاله: بهداشت و بیماری های آبزیان

نویسندگان

1 گروه بهداشت آبزیان، دانشکده دامپزشکی دانشگاه شهید چمران اهواز، اهواز، ایران

2 گروه علوم درمانگاهی، دانشکده دامپزشکی دانشگاه شهید چمران اهواز ، اهواز، ایران

3 مرکز تحقیقات آبزی پروری ایران، مرکز تحقیقات ذخایر آبزیان داخلی گرگان، گلستان، ایران

4 مرکز تحقیقات دارویی دانشگاه علوم پزشکی مشهد، دانشکده علوم پزشکی، مشهد، ایران

5 گروه پاتوبیولوژی، دانشکده دامپزشکی دانشگاه شهید چمران اهواز ، اهواز، ایران

چکیده

زمینه مطالعه: در آبزی پروری رشد سریع، کارایی مناسب تغذیه و افزایش مقاومت در برابر بیماری‌ها بسیار مطلوب است. یکی از راهکارهای مناسب برای بهبود شاخص‌های رشد و مقاومت ماهی استفاده از باکتری‌های پروبیوتیکی است. هدف: مطالعه حاضر به منظور ارزیابی اثر پروبیوتیکی باکتری لاکتوباسیلوس بولگاریکوس ریزپوشانی شده با نانوذرات آلژینات/کیتوزان بر رشد و کارایی تغذیه در فیل ماهی جوان (Huso huso) انجام گرفت. روش کار: این آزمایش به صورت طرح کاملاً تصادفی در قالب چهار گروه تیمار و یک گروه شاهد هرکدام  با سه تکرار صورت پذیرفت، برای این منظور تعداد 375 قطعه فیل ماهی با میانگین وزنی  gr8/2±2/27 در پنج گروه تقسیم شدند.تیمار‌های آزمایشی شامل: T1 با غذای حاوی آلژینات/کیتوزان بدون باکتری، T2 با غذای حاوی باکتری ریزپوشانی شده به روش امولسیون، T3 با غذای حاوی باکتری ریزپوشانی شده به روش ژلاسیون یونی، T4 با غذای حاوی باکتری بدون پوشش و گروه شاهد با غذای پایه تغذیه شدند. ماهیان روزانه به میزان 3 % وزن بدن تغذیه شدند. نتایج: نتایج نشان دادند که حداکثرمقدار ضریب تبدیل غذایی (FCR) در T3 (11/0±64/1) و کمترین آن درT4 (06/0±14/1) بود. T4 در شاخص‌های SGR، PER، FER، در روز 30 و 60 دارای بهترین عملکرد نسبت به سایر تیمارها بود. نتیجهگیرینهایی: براساس نتایج بدست آمده می‌توان نتیجه گرفت که عملکرد رشد در تیمارهایی که کیتوزان و آلژینات به عنوان پوشش حضور داشتند نسبت به تیمار لاکتوباسیلوس بدون پوشش پایین تر بود.

کلیدواژه‌ها


عنوان مقاله [English]

Comparison effects of Lactobasillos bulgaricus microencapsulated by nano alginat/chitosan on Growth performance and Feed efficiency great sturgeon (Huso huso) juveniles.

نویسندگان [English]

  • Seyed Samad Hosseini 1
  • Mojtaba Alishahi 2
  • Koroush Amini 3
  • Mohammadreza Aabbaspour 4
  • Masoud Ghorbanpoor 5
  • Takavar Mohammadian 2
1 1Department of Clinical Sciences, Faculty of Veterinary Medicine Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 2Department of Clinical Sciences, Faculty of Veterinary Medicine Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 3Inland Waters Aquatics Stocks Research Center Gorgan, Gorgan, Iran
4 Targeted Drug Delivery Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
5 5Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran
چکیده [English]

BACKGROUND: In aquaculture the fast growth, high feed efficiency and increased resistance against pathogens are favorable. One of the suitable approaches for improving the growth indices and resistance against pathogens is the application of probiotic bacteria. OBJECTIVES: The present study was conducted to evaluate the effect of nano/microencapsulated Lactobasillus bulgaricus by nano alginate/chitosan on the growth rate and feeding efficiency of great sturgeon (Huso huso) juveniles. METHODS: This experiment was conducted in a completely random design in four groups and group (5) with triplicate. 375 fish weighing (27.28±2.86 gr mean±SD), were randomly divided in to five equal groups in triplicates. The test groups were as follows: fish in T1 were fed with alginate/chitosan without bacterium, fish in T2 were fed with encapsulated bacteria with emulsification method, fish in T3 were fed with encapsulated bacteria with Ionicgelation method, fish in T4 were fed with only bacteria and control group. The fish were fed  3% of body weight per day. RESULTS: The results showed  the highest feed conversion ratio (FCR) was measured in T3 (1.64±0.11) and the lowest was observed in T4 (1.14±0.06).The highest values of SGR, PER, FER in T4 were seen among  the  treatments at days 30 and 60.                                                                                                                                             CONCLUSIONS: According the result, nanoalginat/Chithosan used for encapsulation of probiotics can be lead to decrease in growth performance compared to the other treatments.

کلیدواژه‌ها [English]

  • Probiotic
  • Nanocapsulate
  • Husu huso
  • Growth parameters
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