تأثیر مکمل بتائین بر بیان ژن و فعالیت آنزیم‏های لیپوژنیک و سطوح لیپیدهای خون و کبد جوجه‎های گوشتی

نوع مقاله: علوم پایه (بیوشیمی، آناتومی، جنین، بافت شناسی و فیزیولوژی)

نویسندگان

1 گروه علوم دامی، واحد بهبهان، دانشگاه آزاد اسلامی، بهبهان، ایران

2 گروه علوم دامی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

3 گروه علوم دامی، واحد ورامین-پیشوا، دانشگاه آزاد اسلامی، ورامین، ایران

چکیده

زمینه مطالعه: بتائین مشتق تریمتیله‏ای ازآمینواسیدگلایسین است که در بدن بسیاری از جانوران یافت می‏شود. هدف: این پژوهش به منظور تعیین اثر افزودن مکمل بتائین در جیره بر بیان ژن و فعالیت آنزیمهای لیپوژنیک و لیپیدهای سرم و کبد در جوجههای گوشتی انجام شد. روش کار: در مجموع 320 قطعه جوجه گوشتی (راس 308) در قالب طرح کاملاً تصادفی با 4 تیمار و 4 تکرار، با جیرههای آزمایشی که شامل تیمار شاهد، بدون مصرف مکمل بتائین، و تیمارهای دوم، سوم و چهارم که به ترتیب شامل 05/0، 08/0 و 11/0٪ بتائین هیدروکلراید بودند، مورد بررسی قرار گرفتند. نتایج: افزودن بتائین به جیره اثر معنیداری بر بیان ژن لیپوپروتئینلیپاز و فعالیت آنزیمهای استیلکوآنزیم A کربوکسیلاز و فتیاسید سینتاز نداشت (0.05<p). افزودن مکمل بتائین سبب کاهش معنیدار کلسترول و تریآسیلگلیسرول در کبد جوجههای تیمار چهارم (حاوی 08/0٪ بتائین هیدروکلراید) نسبت به گروه شاهد گردید (0.05>p). نتیجه‏گیری نهایی: به طور کلی نتایج نشان داد که بیان ژن لیپوپروتئینلیپاز و فعالیت آنزیمهای استیل‏کوآنزیم A کربوکسیلاز و فتیاسیدسینتاز در جوجههای گوشتی، کمتر زیر تأثیر افزودن بتائین به جیره قرار می‏گیرد.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Betaine Supplementation on Gene Expression and Activities of Lipogenic Enzymes, and Lipid Levels in Liver and Blood of Broiler Chicks

نویسندگان [English]

  • Nima Mosavat 1
  • Mohammad Chamani 2
  • Farhad Foroudi 3
  • Aliasghar Sadeghi 2
1 Department of Animal Sciences, Behbahan Branch, Islamic Azad University, Behbahan, Iran
2 2Department of Animal Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 Department of Animal Sciences, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
چکیده [English]

BACKGROUND: Betaine is a derivative of three methylates of glycine amino acids, found in the body of many animals. Objectives: The study was conducted to investigate the effects of betaine supplementation in the diet on gene expression and activities of lipogenic enzymes, and lipid levels of blood and liver in broilers. Methods: A total of 320 broilers were evaluated (Ross 308) in a completely randomized design with 4 treatments and 4 replicates, with the diets consisted of the control treatment which did not use the betaine supplementation. The second, third and fourth treatments contained 0.05, 0.08 and 0.11% betaine hydrochloride 98%, respectively. Results: Betaine supplementation to the diet has no significant effect on lipoprotein lipase gene expression, and the activities of enzyme Acetyl-CoA carboxylase and fatty acid synthase (p> 0.05). Betaine supplementation caused a significant decrease in liver cholesterol and triglyceride chicks fourth treatment (containing betaine hydrochloride 0.11 percent) compared to the control group (p<0.05). Conclusions: In general, the results show that in broilers lipoprotein lipase gene expression and the activities of enzyme Acetyl-CoA carboxylase and fatty acid synthase are less influenced by betaine.

کلیدواژه‌ها [English]

  • betaine
  • blood lipids
  • lipogenic enzymes
  • liver lipids
  • gene expression
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