بررسی شیوع سرمی آلودگی به ویروس طاعون نشخوارکنندگان کوچک در گوسفند و گاو دراهواز

نوع مقاله: بهداشت و بیماری های دام های بزرگ

نویسندگان

1 گروه مدیریت بهداشت دام، دانشکده دامپزشکی، دانشگاه شیراز، شیراز، ایران؛ قبلاً، بخش بیماریهای داخلی دامهای بزرگ‏، دانشکده دامپزشکی، دانشگاه شهیدچمران اهواز، اهواز، ایران

2 بخش بیماریهای داخلی دامهای بزرگ، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

3 بخش ویروس شناسی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

4 دانش آموخته دامپزشکی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

5 بخش ویروس شناسی‏، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

چکیده

 
زمینه مطالعه: طاعون نشخوار کنندگان کوچک یک بیماری حاد و به شدت واگیردار نشخوارکنندگان کوچک می‌باشد. این بیماری با تب بالا، ترشحات چشم و بینی، پنومونی، نکروز و اولسر غشاهای مخاطی و التهاب دستگاه گوارش که به اسهال شدید ختم می‌شود، مشخص می‌گردد.
هدف: هدف از انجام این مطالعه بررسی شیوع سرمی آلودگی به ویروس طاعون نشخوارکنندگان کوچک درگوسفندان و گاوهای شهرستان اهواز بود.
روش کار: از 100رأس گوسفند و 100 رأس گاو که در مجاور هم نگه‌داری می‌شدند، از مناطق مختلف شهرستان اهواز خون‌گیری به عمل آمد. همچنین از 16 راس گوسفند که واکسن طاعون نشخوار کنندگان کوچک را دریافت کرده بودند به عنوان کنترل مثبت خون‌گیری شد. سرم‌ها توسط سانتریفیوژ با دور 3000 به مدت 10 دقیقه جدا ساز‌ی شدند و ml 3  از سرم‌ها تا زمان جداسازی آنتی‌بادی ضد ویروسPPR به روش آزمون خنثی‌سازی ویروس، در دمای C° 20- ذخیره گردید.
نتایج: شیوع سرمی آنتی‌بادی PPR در گوسفند 58 ٪ و در گاو 23 ٪ بود و تمام نمونه‌های گوسفند جمع‌آوری شده برای کنترل، مثبت بودند.
نتیجهگیری نهایی: آنتی‌بادی‌های جدا شده درگاو و گوسفند، انتقال طبیعی ویروس را تحت شرایط فیلد تأیید کرد و اطلاعاتی پایه در خصوص شیوع سرمی آنتی‌بادی‌های ویروس PPR در جمعیت گاو و گوسفند در شهرستان اهواز فراهم نمود.

کلیدواژه‌ها


عنوان مقاله [English]

Seroprevalence of Peste Des Petits Ruminants (PPR) Virus Infection in Sheep and Cattle in Ahvaz

نویسندگان [English]

  • Aria Rasooli 1
  • Mohammad Nouri 2
  • Masoud-Reza Seyfi Abadshapouri 3
  • Elham Khalafi 4
  • Maryam Daghari 5
1 1Department of Animal Health Management, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran; formerly, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 2Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4 4Graduated of Veterinary Medicine, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
5 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz
چکیده [English]

BACKGROUND: Peste des petits ruminants (PPR) is an acute and highly contagious viral disease of small ruminants that is characterized by high fever, ocular and nasal discharge, pneumonia, necrosis, ulceration of the mucous membranes and inflammation of the gastro-intestinal tract leading to severe diarrhea.
OBJECTIVES: The aim of this study was to investigate the seroprevalence of peste des petits ruminants (PPR) virus infection in sheep and cattle in Ahvaz.
METHODS: Blood samples were taken from 100 cattle and 100 sheep that were kept together from different parts of Ahvaz. Blood samples were also taken from 16 vaccinated sheep   against PPR for positive control. The sera were separated by centrifuge at 3000 ×g for 10 minutes and 3 mL of serum was harvested and stored at -20 °C until determination of antibody against PPR using VN method.
RESULTS: The peste des petits ruminants (PPR) antibody seroprevalence was 23% in cattle and 58% in sheep and all the sheep samples collected for control were positive for PPR antibody.
CONCLUSIONS: The present study indicates serological evidence for the natural transmission of PPRV from sheep to cattle under natural conditions and provides baseline information on prevalence of PPRV antibodies in cattle and sheep population in Ahvaz.

کلیدواژه‌ها [English]

  • Cattle
  • sheep
  • PPR
  • Seroprevalence
  • virus
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