بررسی فراوانی حامل‌های ویروس تب برفکی در گوسفندان ذبحی کشتارگاه صنعتی مشهد با استفاده از RT-PCR

نویسندگان

1 موسسه تحقیقات واکسن و سرم سازی رازی شعبه شمال شرق، بخش تحقیقات دامپزشکی و بیوتکنولوژی، مشهد–ایران

2 دانش آموخته گروه بیوشیمی، دانشگاه پیام نور مشهد، مشهد –ایران

3 بخش بررسی‌ها، اداره کل دامپزشکی استان خراسان رضوی، مشهد –ایران

چکیده

زمینه مطالعه:‌ بیماری تب برفکی در ایران بومی می‌باشد. روش‌های تشخیصی مولکولی توان بالقوه مهمی برای تشخیص دام‌هایی که دارای عفونت پنهان بوده و یا حامل می‌‌باشند، ارائه نموده است. هدف: بررسی فراوانی حامل‌های ویروس تب برفکی در گوسفندان ذبحی کشتارگاه صنعتی مشهد با استفاده از‌ RT-PCR‌. روش کار:‌ ‌پس از جداسازی 94نمونه از لوزه گوسفندان، واکنش ‌RT-PCR‌ برای تشخیص تب برفکی و تعیین سروتیپ ویروس تب برفکی انجام گردید و در انتها تعیین توالی اسید نوکلئیک انجام شد. نتایج: ‌نتایج به دست آمده از تشخیص ویروس تب برفکی نشان داد 23 نمونه (5/24 )% از نظر وجود ‌RNA‌ ویروس تب برفکی مثبت می‌باشند که از این تعداد 20 نمونه تیپ ‌O‌ تشخیص داده شد و 3 نمونه از نمونه‌های مثبت از نظر تب برفکی به تعیین تیپ با پرایمر‌های اختصاصی جواب ندادند، همچنین نتایج حاصل تعیین توالی اسید نوکلئیک ناحیه‌ D1‌ ژنــوم ویــروس نـشــان داد کـه ویـروس تـب بـرفکـی حـامـل گـوسفنـدی ‌Sheep)2010/100(O/IRN/‌ دارای 02/92% تشـابـه بـا ویـروس                                                                                                         2005-2001/67O/IRN/‌ و42/88% تشابه با ویروس‌ 2008-2004/15O/IRN/‌ می‌باشد. نتیجه‌گیری نهایی: مطالعه حاضر نشان داد که در صـد گـوسـفـنـدان حـاملـیـن ویـروس تـب بـرفکی در کشتارگاه مشهد قابل توجه می‌باشد. توصیه می‌گردد تا ازبر آورد دوره‌ای حاملین در گاو و نشخوارکنندگان کوچک  بعنوان آگاهی دهنده از برنامه‌های کنترلی در منطقه استفاده گردد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluate the frequency of foot and mouth disease viral carriers in slaughtered sheep in Mashhad industrial abattoir using RT-PCR

نویسندگان [English]

  • Saeid Zibaee 1
  • Samad Rezaee 2
  • Mohammad Rashtibaf 3
1 Department of Veterinary Research and Biothecnology, Razi vaccine and Serum Research Institute, North East Branch, Mashhad-Iran
2 Graduated from the Payam-e-Noor University of Mashhad, Department of Biochemistry, Mashhad-Iran
3 Department of Investigation, khorasan-Razavi Provincial Veterinary Service, Mashhad-Iran
چکیده [English]

BACKGROUND: Foot -and- mouth disease (FMD) is endemic in Iran. Molecular techniques for diagnosis of persistent infection or carrier animals have shown a potential ability to improve the detection of a low genome copy number in samples. Objectives: The purpose of this study is to evaluate the frequency of foot and mouth disease viral carriers in slaughtered sheep in Mashhad industrial abattoir using RT-PCR. Methods: Samples were isolated from tonsil of 94 slaughtered sheep analyzed by RT-PCR experiment for the detection of FMD, identification of FMD virus serotypes and at the end nucleic acid sequencing were performed. Results: The results showed that the 23 samples (24/5 percent) were positive for the presence of FMD virus RNA, of which 89.9% of cases are type O and 3 cases of FMD samples did not respond. The results of the 1D genome sequencing of the nucleic acid virus showed that FMD virus of sheep (O/IRN/100/2010Sheep), has 92/02% similarity with the virus (O/IRN/67/2001-2005) and 88/42% similarity with the virus (O/IRN/15/2004-2008). Conclusions: This study showed that the percentage of FMDV sheep carriers in Mashhad slaughterhouse was remarkable. Estimation of the frequency of carrier state in cattle and small ruminants is recommended as a monitor of control plan in the region.

کلیدواژه‌ها [English]

  • FMD virus
  • Molecular detection
  • sheep carriers
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